Tumour-associated antigen (TAA)-specific cytotoxic T cell (CTL) response in vitro and in a mouse model, induced by TAA-plasmids delivered by influenza virosomes

Eur J Cancer. 2001 Nov;37(16):2097-103. doi: 10.1016/s0959-8049(01)00241-6.

Abstract

We investigated influenza virosomes as a TAA-gene delivery system for use in TAA-directed anti-cancer vaccine therapy. An engineered plasmid (GC90) expressing the parathyroid hormone-related peptide (PTH-rP), a protein secreted by prostate and lung carcinoma cells, was included in influenza virosomes (GC90V). The ability of GC90V to elicit a PTH-rP-specific cytotoxic T cell (CTL) response was demonstrated in BALB/c mice immunised with intranasal (i.n.) GC90V+/-adjuvant subcutaneous (s.c.) interleukin-2 (IL-2). A PTH-rP-specific CTL response with antitumour activity was also demonstrated in human peripheral blood mononuclear cells (PBMC) stimulated in vitro with GC90V infected autologous dendritic cells (DC). These results provide a rationale for investigating GC90V in clinical trials of anticancer vaccine therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intranasal
  • Animals
  • Antigens, Neoplasm / genetics
  • Antigens, Neoplasm / immunology*
  • Cancer Vaccines / administration & dosage*
  • Cancer Vaccines / immunology
  • Cell Culture Techniques
  • Cytotoxicity, Immunologic / immunology*
  • Dendritic Cells / immunology
  • Female
  • Gene Transfer Techniques*
  • Humans
  • Influenza A virus / genetics
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / immunology
  • Parathyroid Hormone-Related Protein
  • Plasmids
  • Proteins / genetics
  • Proteins / immunology
  • T-Lymphocytes, Cytotoxic / immunology*
  • Transfection / methods
  • Tumor Cells, Cultured
  • Virosomes

Substances

  • Antigens, Neoplasm
  • Cancer Vaccines
  • Neoplasm Proteins
  • PTHLH protein, human
  • Parathyroid Hormone-Related Protein
  • Proteins
  • Virosomes