A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase

H Uchida; H Morinaga; T Misaki; T Miyazaki; T Uwajima; T Obata; Y Endo; A Matsuda; T Sasaki

doi:10.1081/NCN-100105901

A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase

Nucleosides Nucleotides Nucleic Acids. 2001 Sep;20(9):1647-54. doi: 10.1081/NCN-100105901.

Authors

H Uchida¹, H Morinaga, T Misaki, T Miyazaki, T Uwajima, T Obata, Y Endo, A Matsuda, T Sasaki

Affiliation

¹ Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Japan. uchida@acbio.acbio.fukui-u.ac.jp

PMID: 11580191
DOI: 10.1081/NCN-100105901

Abstract

By affinity chromatography with Sepharose coupled to 2'-deoxy-1-beta-D-ribofuranosyl-N4-dodecanoylcytosine, deoxycytidine kinase and cytidine deaminase were purified 1,950- and 2,240-fold, respectively, from Ehrlich carcinoma cells, and their enzyme activities for several deoxycytidine analogs were investigated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carcinoma, Ehrlich Tumor / enzymology
Chromatography, Affinity / methods*
Chromatography, High Pressure Liquid
Cytidine Deaminase / isolation & purification*
Cytidine Deaminase / metabolism
Deoxycytidine Kinase / isolation & purification*
Deoxycytidine Kinase / metabolism
Kinetics
Mice
Sepharose / analogs & derivatives
Sepharose / metabolism
Tumor Cells, Cultured

Substances

Sepharose
Deoxycytidine Kinase
Cytidine Deaminase