Abstract
A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to equine herpesvirus 2 in serum samples of horses. By measuring the binding to a single epitope, this blocking ELISA gives a good picture of the antibody status in the animal. The test is based on a monoclonal antibody with neutralizing activity and had a sensitivity of 94% and a specificity of 100%. Antibodies due to newly acquired infection in foals were successfully detected with this blocking ELISA.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Antibodies, Viral / analysis*
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Cell Culture Techniques
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Enzyme-Linked Immunosorbent Assay / methods
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Enzyme-Linked Immunosorbent Assay / veterinary*
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Female
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Herpesviridae Infections / diagnosis
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Herpesviridae Infections / immunology
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Herpesviridae Infections / veterinary*
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Horses
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Male
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Rabbits
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Rhadinovirus / immunology*
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Sensitivity and Specificity
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Tumor Virus Infections / diagnosis
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Tumor Virus Infections / immunology
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Tumor Virus Infections / veterinary*
Substances
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Antibodies, Monoclonal
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Antibodies, Viral