The use of a neutralizing monoclonal antibody to detect infections of equine herpesvirus type 2 (EHV-2)

J Vet Diagn Invest. 2001 Sep;13(5):389-93. doi: 10.1177/104063870101300504.

Abstract

A blocking enzyme-linked immunosorbent assay (ELISA) was developed to detect antibodies to equine herpesvirus 2 in serum samples of horses. By measuring the binding to a single epitope, this blocking ELISA gives a good picture of the antibody status in the animal. The test is based on a monoclonal antibody with neutralizing activity and had a sensitivity of 94% and a specificity of 100%. Antibodies due to newly acquired infection in foals were successfully detected with this blocking ELISA.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antibodies, Viral / analysis*
  • Cell Culture Techniques
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Female
  • Herpesviridae Infections / diagnosis
  • Herpesviridae Infections / immunology
  • Herpesviridae Infections / veterinary*
  • Horses
  • Male
  • Rabbits
  • Rhadinovirus / immunology*
  • Sensitivity and Specificity
  • Tumor Virus Infections / diagnosis
  • Tumor Virus Infections / immunology
  • Tumor Virus Infections / veterinary*

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral