Isolation and structure of five new cancer cell growth inhibitory bufadienolides from the Chinese traditional drug Ch'an Su

J Nat Prod. 2001 Sep;64(9):1148-52. doi: 10.1021/np0101088.

Abstract

Five new bufadienolides, 3beta-formyloxyresibufogenin (1), 19-oxobufalin (2), 19-oxodesacetylcinobufagin (3), 6alpha-hydroxycinobufagin (4), and 1beta-hydroxybufalin (5), have been isolated together with the previously known bufadienolides 6-20 from the Chinese traditional drug "Ch'an Su". The structures were elucidated employing spectroscopic methods. Bufadienolides 1-5 provided significant inhibitory activity against the KB and HL-60 cancer cell lines. In addition, bufadienolide 1 was found active against the MH-60 cancer cell line.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / isolation & purification*
  • Antineoplastic Agents / pharmacology
  • Bufanolides / chemistry
  • Bufanolides / isolation & purification*
  • Bufanolides / pharmacology
  • Bufonidae
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • HL-60 Cells / drug effects
  • Humans
  • KB Cells / drug effects
  • Leukemia, Myeloid
  • Magnetic Resonance Spectroscopy
  • Medicine, Chinese Traditional
  • Mice
  • Molecular Conformation
  • Molecular Structure
  • Nasopharyngeal Neoplasms
  • Skin / metabolism
  • Spectrophotometry, Infrared
  • Spectrophotometry, Ultraviolet
  • Tumor Cells, Cultured / drug effects

Substances

  • Antineoplastic Agents
  • Bufanolides