Abstract
Cyclohexene nucleic acids (CeNA) were synthesized using classical phosporamidite chemistry. Incorporation of a cyclohexene nucleo-side in a DNA chain leads to an increase in stability of the DNA/RNA duplex. CeNA is stable against degradation in serum. A CeNA/RNA hybrid is able to activate E. Coli RNase H. resulting in cleavage of the RNA strand.
MeSH terms
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Circular Dichroism
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Cyclohexanes / chemistry*
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Cyclohexanes / metabolism
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Cyclohexenes
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DNA / chemistry
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Enzyme Activation
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Escherichia coli / enzymology
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Nucleic Acids / chemistry*
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Nucleic Acids / metabolism
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RNA / chemistry*
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RNA / metabolism
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Ribonuclease H / metabolism*
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Sugar Alcohols / chemistry
Substances
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Cyclohexanes
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Cyclohexenes
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Nucleic Acids
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Sugar Alcohols
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cyclohexene
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RNA
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DNA
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Ribonuclease H