Cyclohexene nucleic acids (CeNA) form stable duplexes with RNA and induce RNase H activity

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):785-8. doi: 10.1081/NCN-100002430.

Abstract

Cyclohexene nucleic acids (CeNA) were synthesized using classical phosporamidite chemistry. Incorporation of a cyclohexene nucleo-side in a DNA chain leads to an increase in stability of the DNA/RNA duplex. CeNA is stable against degradation in serum. A CeNA/RNA hybrid is able to activate E. Coli RNase H. resulting in cleavage of the RNA strand.

MeSH terms

  • Circular Dichroism
  • Cyclohexanes / chemistry*
  • Cyclohexanes / metabolism
  • Cyclohexenes
  • DNA / chemistry
  • Enzyme Activation
  • Escherichia coli / enzymology
  • Nucleic Acids / chemistry*
  • Nucleic Acids / metabolism
  • RNA / chemistry*
  • RNA / metabolism
  • Ribonuclease H / metabolism*
  • Sugar Alcohols / chemistry

Substances

  • Cyclohexanes
  • Cyclohexenes
  • Nucleic Acids
  • Sugar Alcohols
  • cyclohexene
  • RNA
  • DNA
  • Ribonuclease H