Abstract
One anticapsid (p26) mouse monoclonal antibody was developed after immunization with recombinant p26 Gag protein and was tested for reactivity with different HIV-1 and HIV-2 isolates by ELISA and Western blot analysis. This antibody, named R26.1, reacted with all HIV-2 isolates tested and with recombinant p26 proteins, but no HIV-1 isolates. The epitope of antibody R26.1 was mapped to residues 50-71 in the N-terminal domain of the capsid protein, a highly conserved region in all HIV-2 isolates sequenced to date. This monoclonal antibody may be useful for the detection of HIV-2, and for the discrimination between HIV-1 and HIV-2 infections. Likewise, the identified epitope may be useful for the detection of p26 antibodies in HIV-2-infected individuals.
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / biosynthesis*
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Antibodies, Monoclonal / genetics
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Antibodies, Monoclonal / immunology*
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Antibody Specificity
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Blotting, Western
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Enzyme-Linked Immunosorbent Assay
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Epitope Mapping
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Epitopes / chemistry
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Epitopes / immunology*
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Gene Products, gag / chemistry
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Gene Products, gag / immunology*
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HIV Antibodies / biosynthesis
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HIV Antibodies / genetics
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HIV Antibodies / immunology*
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HIV Antigens / chemistry
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HIV Antigens / immunology*
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HIV-1 / immunology
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HIV-2 / immunology*
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Humans
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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gag Gene Products, Human Immunodeficiency Virus
Substances
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Antibodies, Monoclonal
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Epitopes
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Gene Products, gag
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HIV Antibodies
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HIV Antigens
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gag Gene Products, Human Immunodeficiency Virus
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gag protein p26, Human immunodeficiency virus