The ability of Halobacterium vallismortis, Halobacterium mediterranei and Halobacterium marismortui (Ginzburg strain) to grow anaerobically and denitrify was determined. Each organism grew anaerobically only in the presence of nitrate. H. marismortui produced nitrite and dinitrogen from nitrate during exponential growth. However, as the culture entered stationary phase, dinitrogen production ceased and nitrous oxide was detected. H. vallismortis produced nitrous oxide and dinitrogen during exponential growth, with dinitrogen production ceasing at the onset of stationary phase. H. mediterranei produced dinitrogen during exponential growth and did not produce nitrous oxide. These results confirm the occurrence of denitrification in the halobacteria.