Role of Janus kinase 3 in mast cell-mediated innate immunity against gram-negative bacteria

Immunity. 2001 Aug;15(2):313-21. doi: 10.1016/s1074-7613(01)00184-4.

Abstract

Mast cells play a pivotal role in innate host immune response to gram-negative bacteria. We report that Janus kinase 3 plays a role in mast cell-mediated bacterial clearance and neutrophil recruitment by regulating the release of tumor necrosis factor from mast cells. The role of JAK3 in mast cell-facilitated neutrophil recruitment and bacterial clearance was investigated by comparing the neutrophil influxes and bacterial clearance in mast cell-deficient W/W(v) mice reconstituted with JAK3(+/+) or JAK(-/-) mast cells. The neutrophil influx, bacterial clearance, and survival outcome in W/W(v) mice reconstituted with JAK3(+/+) mast cells was better than in W/W(v) mice reconstituted with JAK3(-/-) mast cells. These findings provide evidence that JAK3 is a key regulator of mast cell-mediated innate immunity against gram-negative bacteria.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD
  • CD48 Antigen
  • Chemotaxis, Leukocyte
  • Escherichia coli Infections / immunology*
  • Escherichia coli Infections / mortality
  • Gram-Negative Bacterial Infections / immunology
  • Immunity, Innate
  • Janus Kinase 3
  • Mast Cells / immunology*
  • Mast Cells / microbiology
  • Mice
  • Mice, Knockout
  • Neutrophils / immunology*
  • Peritonitis / immunology*
  • Peritonitis / mortality
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / metabolism*
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Antigens, CD
  • CD48 Antigen
  • Tumor Necrosis Factor-alpha
  • Protein-Tyrosine Kinases
  • Jak3 protein, mouse
  • Janus Kinase 3