It was paid attention that CH4 and N2O emission from agricultural soil in Yangtze River Delta where there is most advanced agricultural region in China. In this paper, a regional-scale biogeochemical model DNDC was validated on available field measurement. Then it was used to estimate CH4 and N2O emission in Yangtze River Delta to be 1.69 (1.29-2.09) Tg.a-1 and 0.019 (0.014-0.024) Tg.a-1, respectively. They are about 16.7% and 6.1% of the national total CH4 and N2O emissions from agricultural ecosystems, respectively.