Abstract
Mycoplasma agalactiae, the causative agent of contagious agalactia in small ruminants, produces a protein, named P80, that is detectable in all wild-type isolates examined to date and that appears expressed during the early phase of infection. We describe here the identification, cloning and expression of the gene encoding P80 (ma-mp81). The deduced amino acid sequence is consistent with a hydrophobic and basic protein that possesses a lipoprotein signal peptide. Sequence analysis of gene ma-mp81 suggests that P80 is a membrane lipoprotein that shows significant homology with other putative lipoproteins of M. pneumoniae. An internal 1-kb fragment of ma-mp81 was expressed in Escherichia coli as a 6xHis-tagged protein. The purified recombinant protein greatly reacted with polyclonal anti-P80 sera raised in lamb.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigens, Bacterial / chemistry
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Antigens, Bacterial / genetics
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Antigens, Bacterial / isolation & purification
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics*
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Bacterial Proteins / isolation & purification
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Base Sequence
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Carrier Proteins / chemistry*
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Carrier Proteins / genetics*
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Carrier Proteins / isolation & purification
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Cloning, Molecular
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Escherichia coli / genetics
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Genes, Bacterial / genetics
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Membrane Proteins / chemistry*
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Membrane Proteins / genetics*
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Membrane Proteins / isolation & purification
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Molecular Sequence Data
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Molecular Weight
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Mycoplasma / genetics*
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Recombinant Proteins
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Sequence Analysis, DNA
Substances
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Antigens, Bacterial
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Bacterial Proteins
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Carrier Proteins
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Membrane Proteins
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P80 protein, Mycoplasma
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Recombinant Proteins