Formation of HIV-1 envelope-hepatitis B core antigen hybrids with high affinity for CD4

Biochem Biophys Res Commun. 2001 Jul 20;285(3):639-43. doi: 10.1006/bbrc.2001.5227.

Abstract

We have identified an acceptor site on HIV gp120, where foreign protein sequences can be inserted while retaining the native conformation of gp120. The resulting hybrids showed dual antigenicity, normal glycosylation, and high affinity binding of the CD4 receptor. This site allows insertion of highly immunogenic proteins such as core antigen of hepatitis B virus. By combining the immunogenicity of the carrier protein with the antigenicity of gp120, these hybrids may lead to modified HIV-1 antigens with enhanced immunogenicity.

MeSH terms

  • AIDS Vaccines / genetics*
  • AIDS Vaccines / immunology
  • Animals
  • Binding Sites
  • Blotting, Western
  • CD4 Antigens / metabolism*
  • CD4 Immunoadhesins / metabolism
  • Chromatography, Affinity
  • Dose-Response Relationship, Immunologic
  • Gene Expression
  • HIV Envelope Protein gp120 / genetics*
  • Hepatitis B Core Antigens / genetics*
  • Hepatitis B Core Antigens / metabolism
  • Mice
  • Mice, Inbred Strains
  • Protein Binding
  • Protein Structure, Tertiary / genetics
  • Recombinant Fusion Proteins / genetics*
  • Recombinant Fusion Proteins / immunology
  • Recombinant Fusion Proteins / metabolism*
  • Vaccines, Synthetic / genetics
  • Vaccines, Synthetic / immunology
  • Vaccines, Synthetic / metabolism
  • Vaccinia virus / genetics

Substances

  • AIDS Vaccines
  • CD4 Antigens
  • CD4 Immunoadhesins
  • HIV Envelope Protein gp120
  • Hepatitis B Core Antigens
  • Recombinant Fusion Proteins
  • Vaccines, Synthetic