Abstract
Expression in Escherichia coli of the Myxococcus xanthus gene celA, which encodes an extracellular endoglucanase, resulted in CelA being distributed between cytoplasm, periplasm and membrane. The presence of an adjacent open reading frame downstream from the full celA gene, or the absence of a putative lipoprotein signal sequence, confined CelA distribution to the periplasm and membrane, or to the cytoplasm and periplasm, respectively.
MeSH terms
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Amino Acid Sequence
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Cellulase / genetics*
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Cellulase / metabolism
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Cloning, Molecular
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Gene Expression Regulation, Enzymologic
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Myxococcus xanthus / chemistry
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Myxococcus xanthus / enzymology
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Myxococcus xanthus / genetics*
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Open Reading Frames
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Plasmids
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Protein Sorting Signals
Substances
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Membrane Proteins
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Protein Sorting Signals
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CelA endoglucanase
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Cellulase