Abstract
Periodic accumulation and destruction of mitotic cyclins are important for the initiation and termination of M phase. It is known that both APC(Cdc20) and APC(Hct1) collaborate to destroy mitotic cyclins during M phase. Here we show that this relationship between anaphase-promoting complex (APC) and Clb proteins is reversed in S phase such that the early Clb kinases (Clb3, Clb4, and Clb5 kinases) inactivate APC(Hct1) to allow Clb2 accumulation. This alternating antagonism between APC and Clb proteins during S and M phases constitutes an oscillatory system that generates undulations in the levels of mitotic cyclins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anaphase-Promoting Complex-Cyclosome
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Blotting, Northern
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Blotting, Western
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Cdc20 Proteins
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Cdh1 Proteins
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Cell Cycle Proteins / metabolism
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Cyclin B / metabolism*
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Cyclins / metabolism*
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Flow Cytometry
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Fungal Proteins / metabolism
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Gene Deletion
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Ligases / metabolism
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Microscopy, Fluorescence
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Mitosis*
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Models, Biological
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Mutation
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Phosphorylation
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Plasmids / metabolism
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Protein Binding
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S Phase*
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Saccharomyces cerevisiae Proteins*
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Time Factors
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Transcription, Genetic
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Ubiquitin-Protein Ligase Complexes*
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Ubiquitin-Protein Ligases
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Yeasts / metabolism
Substances
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CDC20 protein, S cerevisiae
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CDH1 protein, S cerevisiae
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CLB2 protein, S cerevisiae
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Cdc20 Proteins
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Cdh1 Proteins
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Cell Cycle Proteins
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Cyclin B
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Cyclins
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Ubiquitin-Protein Ligase Complexes
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Anaphase-Promoting Complex-Cyclosome
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Ubiquitin-Protein Ligases
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Ligases