Early expressed Clb proteins allow accumulation of mitotic cyclin by inactivating proteolytic machinery during S phase

Mol Cell Biol. 2001 Aug;21(15):5071-81. doi: 10.1128/MCB.21.15.5071-5081.2001.

Abstract

Periodic accumulation and destruction of mitotic cyclins are important for the initiation and termination of M phase. It is known that both APC(Cdc20) and APC(Hct1) collaborate to destroy mitotic cyclins during M phase. Here we show that this relationship between anaphase-promoting complex (APC) and Clb proteins is reversed in S phase such that the early Clb kinases (Clb3, Clb4, and Clb5 kinases) inactivate APC(Hct1) to allow Clb2 accumulation. This alternating antagonism between APC and Clb proteins during S and M phases constitutes an oscillatory system that generates undulations in the levels of mitotic cyclins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaphase-Promoting Complex-Cyclosome
  • Blotting, Northern
  • Blotting, Western
  • Cdc20 Proteins
  • Cdh1 Proteins
  • Cell Cycle Proteins / metabolism
  • Cyclin B / metabolism*
  • Cyclins / metabolism*
  • Flow Cytometry
  • Fungal Proteins / metabolism
  • Gene Deletion
  • Ligases / metabolism
  • Microscopy, Fluorescence
  • Mitosis*
  • Models, Biological
  • Mutation
  • Phosphorylation
  • Plasmids / metabolism
  • Protein Binding
  • S Phase*
  • Saccharomyces cerevisiae Proteins*
  • Time Factors
  • Transcription, Genetic
  • Ubiquitin-Protein Ligase Complexes*
  • Ubiquitin-Protein Ligases
  • Yeasts / metabolism

Substances

  • CDC20 protein, S cerevisiae
  • CDH1 protein, S cerevisiae
  • CLB2 protein, S cerevisiae
  • Cdc20 Proteins
  • Cdh1 Proteins
  • Cell Cycle Proteins
  • Cyclin B
  • Cyclins
  • Fungal Proteins
  • Saccharomyces cerevisiae Proteins
  • Ubiquitin-Protein Ligase Complexes
  • Anaphase-Promoting Complex-Cyclosome
  • Ubiquitin-Protein Ligases
  • Ligases