Cg-TIMP, an inducible tissue inhibitor of metalloproteinase from the Pacific oyster Crassostrea gigas with a potential role in wound healing and defense mechanisms(1)

FEBS Lett. 2001 Jun 29;500(1-2):64-70. doi: 10.1016/s0014-5793(01)02559-5.

Abstract

We have cloned and characterized a cDNA encoding Cg-TIMP, the first tissue inhibitor of metalloproteinase identified in mollusks. The isolated cDNA encodes a protein of 221 residues that has a domain organization similar to that of vertebrate TIMPs including a signal sequence, and the 12 cysteines characteristic of the TIMP signature. Analysis of Cg-TIMP expression in adult oyster tissues, by Northern blot and in situ hybridization, indicates that Cg-TIMP was only expressed in hemocytes which are the key components of defense mechanisms in mollusks. We also observed that Cg-TIMP mRNA accumulated during shell damage and bacterial challenge. This pattern of expression suggests that Cg-TIMP may be an important factor in wound healing and defense mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Enzyme Induction
  • Hemocytes / enzymology*
  • Hemocytes / microbiology
  • Molecular Sequence Data
  • Ostreidae / enzymology*
  • Ostreidae / microbiology
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Tissue Inhibitor of Metalloproteinases / biosynthesis
  • Tissue Inhibitor of Metalloproteinases / genetics*
  • Tissue Inhibitor of Metalloproteinases / pharmacology
  • Wound Healing / drug effects
  • Wound Healing / physiology*

Substances

  • DNA, Complementary
  • RNA, Messenger
  • TIMP protein, Crassostrea gigas
  • Tissue Inhibitor of Metalloproteinases

Associated data

  • GENBANK/AF321279