As a result of an investigation of metazoan parasites of elasmobranch fishes in the Gulf of Gabès, Tunisia, we discovered 2 new species of diphyllidean cestodes. Macrobothridium euterpes n. sp. is described from the spiral intestine of Rhinobatos rhinobatos, and Macrobothridium syrtensis n. sp. from the spiral intestine of Rhinobatos cemiculus. Macrobothridium euterpes is distinguished from the only other species in the genus (Macrobothridium rhynchobati) by the number of rostellar hooks, size, genital pore position, vagina position, and ovary shape. Macrobothridium syrtensis is distinguished from M. rhynchobati by the hook morphology, testis number, and overall size, and from M. euterpes by the number of rostellar hooks, testis number, genital pore position, vagina position, and ovary shape. These are the first 2 species added to the genus since its establishment in 1989. A standardized formula for representing the number and arrangement of rostellar hooks in diphyllidean species is presented.