Abstract
Recent studies identified YidC as a novel membrane factor that may play a key role in membrane insertion of inner membrane proteins (IMPs), both in conjunction with the Sec-translocase and as a separate entity. Here, we show that the type II IMP FtsQ requires both the translocase and, to a lesser extent, YidC in vivo. Using photo-crosslinking we demonstrate that the transmembrane (TM) domain of the nascent IMP FtsQ inserts into the membrane close to SecY and lipids, and moves to a combined YidC/lipid environment upon elongation. These data are consistent with a crucial role for YidC in the lateral transfer of TM domains from the Sec translocase into the lipid bilayer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / metabolism*
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Bacterial Proteins / metabolism*
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Carrier Proteins / metabolism*
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Cell Membrane / enzymology*
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Cell Membrane / metabolism
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / enzymology
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Escherichia coli / metabolism
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Escherichia coli Proteins*
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Lipid Metabolism
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Membrane Proteins / metabolism*
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Membrane Transport Proteins*
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Models, Biological
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Plasmids / metabolism
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Protein Binding
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Protein Structure, Tertiary
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RNA, Messenger / metabolism
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Ribosomes / metabolism
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SEC Translocation Channels
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SecA Proteins
Substances
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Bacterial Proteins
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Carrier Proteins
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Escherichia coli Proteins
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FtsQ protein, E coli
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Membrane Proteins
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Membrane Transport Proteins
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RNA, Messenger
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SEC Translocation Channels
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SecY protein, E coli
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YIDC protein, E coli
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Adenosine Triphosphatases
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SecA Proteins