Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display

J W Chin; R M Grotzfeld; M A Fabian; A Schepartz

doi:10.1016/s0960-894x(01)00139-1

Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display

Bioorg Med Chem Lett. 2001 Jun 18;11(12):1501-5. doi: 10.1016/s0960-894x(01)00139-1.

Authors

J W Chin¹, R M Grotzfeld, M A Fabian, A Schepartz

Affiliation

¹ Department of Chemistry, Yale University, New Haven, CT 06520-8107, USA.

PMID: 11412969
DOI: 10.1016/s0960-894x(01)00139-1

Abstract

Synthetic genes for avian pancreatic polypeptide (aPP) and for the miniature DNA binding protein PPBR4 were cloned and expressed on the surface of M13 bacteriophage. We anticipate that these constructs will have utility optimizing the properties of miniature proteins based on aPP that result from our previously described protein grafting procedure.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Bacteriophage M13 / metabolism
Cloning, Molecular
DNA-Binding Proteins / chemical synthesis
Drug Design
Methods
Pancreatic Polypeptide / chemistry*
Peptide Fragments / chemistry*
Peptide Library*
Protein Structure, Secondary
Structure-Activity Relationship

Substances

DNA-Binding Proteins
Peptide Fragments
Peptide Library
pancreatic polypeptide, avian
Pancreatic Polypeptide