Abstract
Incubation of chirally deuterated NADPH with 6-deoxyerythronolide B synthase (DEBS) modules 5 and module 6 and analysis of the derived triketide lactones established that the two ketoreductase domains, KR5 and KR6, are both specific for the 4-pro-S hydride of the nicotinamide cofactor.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Bacteria / enzymology
-
Bacteria / metabolism
-
Catalytic Domain
-
Deuterium
-
Erythromycin / analogs & derivatives*
-
Erythromycin / biosynthesis*
-
Erythromycin / chemistry
-
Gas Chromatography-Mass Spectrometry
-
Multienzyme Complexes / chemistry
-
Multienzyme Complexes / metabolism*
-
NADP / metabolism*
-
Oxidation-Reduction
-
Protein Structure, Tertiary
-
Substrate Specificity
Substances
-
Multienzyme Complexes
-
6-deoxyerythronolide B
-
NADP
-
Erythromycin
-
Deuterium