Abstract
A large number of disease-causing bacteria and viruses are being sequenced and PCR is increasingly used for the diagnosis of the diseases. We have designed a multiplex PCR system for hepatitis B virus (HBV), a DNA virus, and hepatitis E virus (HEV), an RNA virus. A modified technique has been standardized for simultaneous extraction of DNA and RNA, followed by a one-step RT-PCR/PCR.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA / blood
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DNA / chemistry*
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DNA / isolation & purification
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DNA Viruses / chemistry*
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Hepatitis B virus / chemistry
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Hepatitis E virus / chemistry
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Humans
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Nucleic Acid Amplification Techniques*
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Polymerase Chain Reaction / methods
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RNA / blood
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RNA / chemistry*
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RNA / isolation & purification
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RNA Viruses / chemistry*
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Reverse Transcriptase Polymerase Chain Reaction