Abstract
NH(4)(+) is the main product of symbiotic nitrogen fixation and the external concentration of combined nitrogen plays a key regulatory role in all the different step of plant-rhizobia interaction. We report the cloning and characterization of the first member of the ammonium transporter family, LjAMT1;1 from a leguminous plant, Lotus japonicus. Sequence analysis reveals a close relationship to plant transporters of the AMT1 family. The wild type and two mutated versions of LjAMT1;1 were expressed and functionally characterized in yeast. LjAMT1;1 is transcribed in roots, leaves and nodules of L. japonicus plants grown under low nitrogen conditions, consistent with a role in uptake of NH(4)(+) by the plant cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cation Transport Proteins*
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Cell Division / drug effects
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Cell Division / genetics
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DNA, Plant / chemistry
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DNA, Plant / genetics
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Exons
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Genes, Plant / genetics*
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Introns
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Methylamines / metabolism
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Methylamines / pharmacology
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Molecular Sequence Data
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Mutation
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Plant Proteins*
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Plants / genetics*
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Plasmids / genetics
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Carrier Proteins
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Cation Transport Proteins
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DNA, Plant
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Methylamines
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Plant Proteins
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ammonium transporters, plant
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methylamine