The solid/liquid separation of suspended, metals-loaded biomass was studied extensively by successfully applying flotation. Industrial non-living biomass samples of different origin have been tried in the laboratory as sorbents of metal ions, with the main stress on Streptomyces rimosus, an effective actinomyces. Flotation was used as the harvesting technique downstream, following the biosorption stage. Important parameters of the investigation were the solution pH, the surfactant type and concentration, the applied modification of biomass, etc. The reuse and recycling of biosorbent following elution was proved possible. As a result cleaned water was produced as underflow of the flotation process.