Objective: To describe a laparoscopic technique for transplanting frozen-banked ovarian tissue.
Design: Case study.
Setting: Academic medical center.
Patient(s): A patient whose ovarian tissue was previously frozen with the slow-freeze technique.
Intervention(s): Microsurgical reconstruction of ovarian cortex and its laparoscopic transplantation to the ovarian fossa.
Main outcome measure(s): Blood flow to the grafts by doppler, follicle development and ovulation by ultrasound, E(2) and progesterone production, and resumption of spontaneous menses.
Result(s): The patient ovulated and menstruated 4 months after the transplant in response to ovarian stimulation with menopausal gonadotropins.
Conclusion(s): Laparoscopic transplantation of frozen-banked ovarian tissue beneath the pelvic peritoneum can restore ovarian function.