Crystallization and preliminary X-ray study of haem-binding protein from the bloodsucking insect Rhodnius prolixus

R A Nagem; J R Brandão Neto; V P Forrer; M H Sorgine; G O Paiva-Silva; H Masuda; R Meneghini; P L Oliveira; I Polikarpov

doi:10.1107/s0907444901004437

Crystallization and preliminary X-ray study of haem-binding protein from the bloodsucking insect Rhodnius prolixus

Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):860-1. doi: 10.1107/s0907444901004437. Epub 2001 May 25.

Authors

R A Nagem¹, J R Brandão Neto, V P Forrer, M H Sorgine, G O Paiva-Silva, H Masuda, R Meneghini, P L Oliveira, I Polikarpov

Affiliation

¹ Gleb Wataghin Physics Institute, State University of Campinas, UNICAMP, Caixa Postal 6165, CEP 13083-970, Campinas, SP, Brazil.

PMID: 11375508
DOI: 10.1107/s0907444901004437

Abstract

Rhodnius haem-binding protein (RHBP) from the bloodsucking insect Rhodnius prolixus, a 15 kDa protein, has been crystallized using polyethylene glycol as a precipitant. X-ray diffraction data have been collected at a synchrotron source. The crystals belong to the space group P4(1(3))2(1)2, with unit-cell parameters a = b = 64.98, c = 210.68 A, and diffract beyond 2.6 A resolution.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carrier Proteins / chemistry*
Crystallization
Crystallography, X-Ray
Heme-Binding Proteins
Hemeproteins / chemistry*
Insect Proteins / chemistry*
Molecular Weight
Protein Conformation
Rhodnius / chemistry*

Substances

Carrier Proteins
Heme-Binding Proteins
Hemeproteins
Insect Proteins