Abstract
C. fulvum, a fungal tomato pathogen, has previously been shown to express a complex family of hydrophobin genes including four class I hydrophobins and one class II hydrophobin. Here we describe a gene for HCf-6, a sixth member of the hydrophobin family and the second class II gene. The protein is predicted to consist of a signal sequence, an N-terminus rich in glycine and asparagine and a C-terminal hydrophobic domain which bears the hall-marks of hydrophobins. In contrast to the previously described class II hydrophobin HCf-5, HCf-6 is expressed in mycelium growing in pure culture and mRNA levels do not increase during sporulation. It is down-regulated by carbon starvation but not by depletion of nitrogen in the growth medium.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cladosporium / chemistry
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Cladosporium / genetics*
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Cladosporium / metabolism
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DNA, Fungal / chemistry
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DNA, Fungal / genetics
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DNA, Fungal / isolation & purification
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Fungal Proteins / chemistry
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Fungal Proteins / genetics*
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Fungal Proteins / isolation & purification
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Gene Expression Regulation, Fungal
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Genes, Fungal
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Phylogeny
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Polymerase Chain Reaction
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RNA, Fungal / chemistry
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RNA, Fungal / genetics
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RNA, Fungal / isolation & purification
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Surface Properties
Substances
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DNA, Fungal
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Fungal Proteins
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RNA, Fungal
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RNA, Messenger
Associated data
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GENBANK/AF251294
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GENBANK/AF251295