Less is more--length reduction of STR amplicons using redesigned primers

P Wiegand; M Kleiber

doi:10.1007/s004140000162

Less is more--length reduction of STR amplicons using redesigned primers

Int J Legal Med. 2001;114(4-5):285-7. doi: 10.1007/s004140000162.

Authors

P Wiegand¹, M Kleiber

Affiliation

¹ Institute of Pathology and Legal Medicine, Department of Legal Medicine, University of Ulm, Prittwitzstrasse 6, 89075 Ulm, Germany.

PMID: 11355413
DOI: 10.1007/s004140000162

Abstract

PCR primers closely flanking the repeat region were redesigned to reduce the amplicon length of the selected STRs down to approximately 100 bp for the shorter alleles (loci HumTH01, D10S2325, DYS19 and DYS391). Highly degraded DNA (e.g. formalin-fixed tissue) and very low amounts of DNA could be more successfully typed using the new redesigned primers compared to the established sequences generating longer amplicons.

Publication types

Comparative Study

MeSH terms

Body Fluids / chemistry
DNA Fingerprinting / methods*
DNA Primers / genetics*
Humans
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Tandem Repeat Sequences / genetics*

Substances

DNA Primers