Kanamycin susceptibility testing of Mycobacterium tuberculosis using Mycobacterium Growth Indicator Tube and a colorimetric method

Antimicrob Agents Chemother. 2001 Jun;45(6):1934-6. doi: 10.1128/AAC.45.6.1934-1936.2001.

Abstract

Two novel systems were evaluated for performing indirect kanamycin susceptibility tests on 72 strains of Mycobacterium tuberculosis. The microplate Alamar blue colorimetric method (breakpoint, 2.5 microg/ml) and the Mycobacterium Growth Indicator Tube (MGIT) system (breakpoint, 5.0 microg/ml) both produced 98.6% agreement when compared with the conventional proportion method performed on 7H10 agar using 5.0 microg of kanamycin/ml. Both systems provided results within an average of 1 week.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antitubercular Agents / pharmacology*
  • Colorimetry
  • Coloring Agents
  • Drug Resistance, Microbial
  • Kanamycin / pharmacology*
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / growth & development
  • Oxazines*
  • Xanthenes*

Substances

  • Antitubercular Agents
  • Coloring Agents
  • Oxazines
  • Xanthenes
  • resazurin
  • Kanamycin