A novel highly informative polyA microsatellite on the telomeric side of the INK4a/ARF locus

Mol Cell Probes. 2001 Jun;15(3):183-5. doi: 10.1006/mcpr.2001.0352.

Abstract

The INK4a/ARF locus encodes two cell cycle-regulatory proteins, p16(INK4a)and p14(ARF). Inactivation of the p16(INK4a)(MTS1) tumor suppressor gene by mutations, promoter methylation or gene deletions is a common event in the development of many different human tumors. The present report describes a novel polyA mononucleotide repeat situated 7.2 kb on the telomeric side of the INK4a/ARF locus. This highly polymorphic microsatellite marker (heterozygote frequency: 0.78) proved to be efficient for p16 allele loss and microsatellite instability analyses in human colon cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics
  • Alleles
  • Colon / pathology
  • Colonic Neoplasms / genetics
  • Cyclin-Dependent Kinase Inhibitor p16 / genetics*
  • Heterozygote
  • Humans
  • Loss of Heterozygosity
  • Microsatellite Repeats*
  • Mucous Membrane / pathology
  • Poly A / genetics*
  • Polymorphism, Genetic
  • Proteins / genetics*
  • Telomere / ultrastructure*
  • Tumor Suppressor Protein p14ARF

Substances

  • Cyclin-Dependent Kinase Inhibitor p16
  • Proteins
  • Tumor Suppressor Protein p14ARF
  • Poly A