Quality control of PCR primers used in multiplex STR amplification reactions

J M Butler; J M Devaney; M A Marino; P M Vallone

doi:10.1016/s0379-0738(00)00412-6

Quality control of PCR primers used in multiplex STR amplification reactions

Forensic Sci Int. 2001 Jun 1;119(1):87-96. doi: 10.1016/s0379-0738(00)00412-6.

Authors

J M Butler¹, J M Devaney, M A Marino, P M Vallone

Affiliation

¹ Biotechnology Division, National Institute of Standards and Technology, 100 Bureau Drive, Mail Stop 8311, Gaithersburg, MD 20899, USA. john.butler@nist.gov

PMID: 11348798
DOI: 10.1016/s0379-0738(00)00412-6

Abstract

Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification reactions where multiple STR loci are simultaneously copied. Commercially available kits are now widely used for STR amplification and subsequent DNA typing. We present here the use of high performance liquid chromatography (HPLC) and time-of-flight mass spectrometry (TOF-MS) methods for characterization of commercially available STR kits.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Validation Study

MeSH terms

Chromatography, High Pressure Liquid / methods*
DNA Primers / standards*
Discriminant Analysis
Forensic Medicine / methods*
Humans
Mass Spectrometry / methods*
Minisatellite Repeats / genetics*
Nucleic Acid Amplification Techniques / methods*
Polymerase Chain Reaction / methods*
Quality Control
Time Factors

Substances

DNA Primers