Abstract
[reaction in text] A new method for the synthesis of selenocysteine derivatives and selenocysteine-containing peptides is described. Fmoc-Se-p-methoxybenzylselenocysteine (1) was prepared and used for solid-phase synthesis of peptides with an N-terminal unprotected selenocysteine. Subsequent native chemical ligation with a peptide thioester provided a 17-mer that corresponds to the C-terminus of ribonucleotide reductase with selenocysteine in place of cysteine.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Peptides / chemical synthesis*
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Peptides / chemistry
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Ribonucleotide Reductases / chemistry
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Selenocysteine / chemical synthesis*
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Selenocysteine / chemistry
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Sequence Analysis, Protein
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Sequence Homology, Amino Acid
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Structure-Activity Relationship
Substances
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Peptides
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Selenocysteine
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Ribonucleotide Reductases