Quantifying DNA damage by gel electrophoresis, electronic imaging and number-average length analysis

Electrophoresis. 2001 Mar;22(5):843-54. doi: 10.1002/1522-2683()22:5<843::AID-ELPS843>3.0.CO;2-9.

Abstract

DNA damages that can be converted to single- or double strand breaks can be quantified by separating DNA by gel electrophoresis and obtaining a quantitative image of the resulting distribution of DNA in the gel. We review the theory of this method and discuss its implementation, including the charge-coupled device (CCD) camera systems we developed to acquire images of fluorophore labeled DNA.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • DNA / analysis
  • DNA / isolation & purification
  • DNA Damage*
  • Electrophoresis, Agar Gel* / instrumentation
  • Electrophoresis, Capillary
  • Fluorescent Dyes
  • Image Processing, Computer-Assisted* / instrumentation
  • Lasers
  • Photography
  • Sensitivity and Specificity

Substances

  • Fluorescent Dyes
  • DNA