Abstract
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly between sera from B. equi-infected horses and sera from Babesia caballi-infected horses or from normal horses. The LAT results were identical to those of a previously developed enzyme-linked immunosorbent assay. These results indicate that LAT using recombinant EMA-1 might be very useful as a routine screening method for the diagnosis of B. equi infection.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Protozoan / immunology*
-
Antigens, Protozoan / genetics
-
Antigens, Protozoan / immunology
-
Babesia / immunology*
-
Babesiosis / diagnosis
-
Babesiosis / immunology*
-
Babesiosis / parasitology
-
Horses
-
Latex Fixation Tests
-
Membrane Proteins / genetics
-
Membrane Proteins / immunology
-
Protozoan Proteins / genetics
-
Protozoan Proteins / immunology
-
Recombinant Proteins / genetics
-
Recombinant Proteins / immunology
Substances
-
Antibodies, Protozoan
-
Antigens, Protozoan
-
EMA-1 protein, Babesia equi
-
Membrane Proteins
-
Protozoan Proteins
-
Recombinant Proteins