Abstract
The aim of this work was to study the regulation of LPS-stimulated PGE 2 synthesis by traditional NSAIDs (piroxicam and diclofenac) and a selective COX-2 inhibitor (NS-398), in cultured bovine corneal endothelial cells and retinal pigmentary epithelial cells. The IC50 values of piroxicam and diclofenac were compared with IC50 values of NS-398, diclofenac, in both types of cells, showed higher potency than piroxicam. Diclofenac seemed to be a COX-2 inhibitor because its IC50 values were similar to the IC50 values of NS-398. We suggest that this in vitro cell assay system could be useful for identifying compounds that selectively inhibit COX-2 in ocular tissues.
MeSH terms
-
Animals
-
Anti-Inflammatory Agents, Non-Steroidal / pharmacology
-
Cattle
-
Cells, Cultured
-
Cyclooxygenase 2
-
Cyclooxygenase 2 Inhibitors
-
Cyclooxygenase Inhibitors / metabolism*
-
Diclofenac / pharmacology
-
Dinoprostone / biosynthesis
-
Endothelium, Corneal / drug effects
-
Endothelium, Corneal / enzymology*
-
Isoenzymes / antagonists & inhibitors*
-
Lipopolysaccharides / pharmacology
-
Nitrobenzenes / pharmacology
-
Pigment Epithelium of Eye / drug effects
-
Pigment Epithelium of Eye / enzymology*
-
Piroxicam / pharmacology
-
Prostaglandin-Endoperoxide Synthases
-
Salmonella typhimurium
-
Sulfonamides / pharmacology
Substances
-
Anti-Inflammatory Agents, Non-Steroidal
-
Cyclooxygenase 2 Inhibitors
-
Cyclooxygenase Inhibitors
-
Isoenzymes
-
Lipopolysaccharides
-
Nitrobenzenes
-
Sulfonamides
-
N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
-
Piroxicam
-
Diclofenac
-
Cyclooxygenase 2
-
Prostaglandin-Endoperoxide Synthases
-
Dinoprostone