A methanol method for the preparation of template DNA for polymerase chain reaction (PCR) from peripheral blood specimens was established. A diameter of 3 mm blood spots on paper were punched and fixed in methanol for overnight. Deionized water was then added. After boiling and centrifuging, the supernatants reserved DNA. The blood blot of specimen is easy to be obtained and reserved in low cost. The experiment technique is high in efficiency.