Identification and characterization of a novel tospovirus species using a new RT-PCR approach

Arch Virol. 2001;146(2):265-78. doi: 10.1007/s007050170174.

Abstract

A novel tospovirus serologically distinct from all established tospovirus species was found in Thailand in Physalis minima L. The S RNA of this virus was cloned by a new RT-PCR approach revealing a nucleotide sequence of 3257 nucleotides. The ambisense RNA segment encoded a nonstructural protein (NSs) of 469 amino acids, with a predicted Mr of 53.2 kDa, and a nucleoprotein (N) of 279 amino acids and a Mr of 31.0 kDa, so far the largest N protein known for any tospovirus species. N protein sequence comparisons revealed closet relationship to the species Watermelon bud necrosis virus (58% identity), Watermelon silver mottle virus and Peanut bud necrosis virus (57%) and a distant relationship to Peanut yellow spot virus (23%) and Peanut chlorotic fanspot virus (22%).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Evolution, Molecular
  • Molecular Sequence Data
  • Molecular Weight
  • Nucleocapsid Proteins / chemistry
  • Nucleocapsid Proteins / genetics*
  • Phylogeny*
  • Polymerase Chain Reaction / methods
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Solanaceae / virology*
  • Species Specificity
  • Thailand
  • Tospovirus / classification*
  • Tospovirus / genetics*
  • Tospovirus / isolation & purification

Substances

  • Nucleocapsid Proteins
  • RNA, Viral