Use of lipophilic anions for estimation of biomass and cell viability

Biotechnol Bioeng. 2000;71(3):208-16. doi: 10.1002/1097-0290(2000)71:3<208::aid-bit1010>3.0.co;2-0.

Abstract

A method is described to estimate the microbial biomass of a sample, to enumerate the cells, and to distinguish the portion of metabolically active cells in the population by measuring the binding of phenyldicarbaundecaborane (PCB(-)) to the cells. This method can also be used for the analysis of a complex population of microorganisms if the cells composing the sample are sensitive to different biocidal agents. In addition, the analysis of PCB(-) binding is useful for the enumeration of the phage-infected cells and phage particles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophages / drug effects
  • Bacteriophages / physiology*
  • Biomass*
  • Boron Compounds* / chemistry
  • Cell Survival / drug effects*
  • Escherichia coli / drug effects
  • Escherichia coli / physiology*
  • Kinetics
  • Models, Theoretical
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / physiology*
  • Spectrum Analysis / methods*

Substances

  • Boron Compounds
  • phenyldicarbaundecaborane