Abstract
A novel human member of the Bcl-2 family was identified, Bcl-B, which is closest in amino acid sequence homology to the Boo (Diva) protein. The Bcl-B protein contains four Bcl-2 homology (BH) domains (BH1, BH2, BH3, BH4) and a predicted carboxyl-terminal transmembrane (TM) domain. The BCL-B mRNA is widely expressed in adult human tissues. The Bcl-B protein binds Bcl-2, Bcl-X(L), and Bax but not Bak. In transient transfection assays, Bcl-B suppresses apoptosis induced by Bax but not Bak. Deletion of the TM domain of Bcl-B impairs its association with intracellular organelles and diminishes its anti-apoptotic function. Bcl-B thus displays a unique pattern of selectivity for binding and regulating the function of other members of the Bcl-2 family.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Apoptosis / physiology*
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism*
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Cell Line
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Cloning, Molecular
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Expressed Sequence Tags
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Humans
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Membrane Proteins / metabolism*
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Mice
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Open Reading Frames
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Organelles / metabolism
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-bcl-2*
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RNA, Messenger / genetics
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Deletion
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Sequence Homology, Amino Acid
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Transcription, Genetic*
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Transfection
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bcl-2 Homologous Antagonist-Killer Protein
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bcl-2-Associated X Protein
Substances
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BAK1 protein, human
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BAX protein, human
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Bak1 protein, mouse
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Bax protein, mouse
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Carrier Proteins
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Membrane Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-bcl-2
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RNA, Messenger
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Recombinant Proteins
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bcl-2 Homologous Antagonist-Killer Protein
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bcl-2-Associated X Protein
Associated data
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GENBANK/AF285092
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GENBANK/AF326964