Characterization of PBZ1, a probenazole-inducible gene, in suspension-cultured rice cells

Biosci Biotechnol Biochem. 2001 Jan;65(1):205-8. doi: 10.1271/bbb.65.205.

Abstract

Probenazole (PBZ) induces non-race specific resistance in rice plants against rice blast fungus and PBZ1 was identified as a PBZ-inducible gene from rice. The induction of PBZ1 expression in suspension-cultured rice cells was investigated. Northern blot analysis indicated that PBZ1 was induced by PBZ in a dose-dependent manner. Enzyme-linked immunosorbent assay (ELISA) showed a dose and time-dependent accumulation of PBZ1 protein. Both mRNA and protein analysis showed that PBZ1 was not induced by salicylic acid or an active metabolite, 1,2-benzisothiazole-1,1-dioxide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Gene Expression Regulation, Plant*
  • Oryza / chemistry
  • Oryza / drug effects
  • Oryza / genetics*
  • Plant Proteins / biosynthesis*
  • Plant Proteins / genetics
  • Salicylic Acid / pharmacology
  • Thiazoles / pharmacology*

Substances

  • PBZ1 protein, Oryza sativa
  • Plant Proteins
  • Thiazoles
  • oryzemate
  • Salicylic Acid