Identification of four genes of the Brucella melitensis ATP synthase operon F0 sector: relationship with the Rhodospirillaceae family

Microb Comp Genomics. 2000;5(3):163-71. doi: 10.1089/omi.1.2000.5.163.

Abstract

We have determined the nucleotide sequence of a cloned DNA fragment from the human and animal pathogen Brucella melitensis. Four genes were identified from a 4069 bp fragment, corresponding to the B. melitensis a, c, b', and b subunits of the ATP synthase F0 sector operon. A duplicated and divergent copy of the b-subunit gene was observed. This feature has been found only in photosynthetic bacteria and chloroplasts. In addition, the gene cluster was separated from the F1 sector, a characteristic described only for the Rhodospirillaceae family.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • ATP Synthetase Complexes
  • Amino Acid Sequence
  • Base Sequence
  • Brucella melitensis / genetics*
  • DNA, Complementary / genetics
  • Genes, Bacterial
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics*
  • Multigene Family
  • Operon
  • Phosphotransferases (Phosphate Group Acceptor) / genetics*
  • Regulatory Sequences, Nucleic Acid
  • Restriction Mapping
  • Rhodospirillaceae / genetics*
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Complementary
  • Multienzyme Complexes
  • ATP Synthetase Complexes
  • Phosphotransferases (Phosphate Group Acceptor)

Associated data

  • GENBANK/AF054609
  • GENBANK/AF054610
  • GENBANK/AF059568