Mammalian inositol polyphosphate multikinase synthesizes inositol 1,4,5-trisphosphate and an inositol pyrophosphate

Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2306-11. doi: 10.1073/pnas.041614598. Epub 2001 Feb 13.

Abstract

Using a consensus sequence in inositol phosphate kinase, we have identified and cloned a 44-kDa mammalian inositol phosphate kinase with broader catalytic capacities than any other member of the family and which we designate mammalian inositol phosphate multikinase (mIPMK). By phosphorylating inositol 4,5-bisphosphate, mIPMK provides an alternative biosynthesis for inositol 1,4,5-trisphosphate [Ins(1,4,5)P(3)]. mIPMK also can form the pyrophosphate disphosphoinositol tetrakisphosphate (PP-InsP(4)) from InsP(5). Additionally, mIPMK forms InsP(4) from Ins(1,4,5)P(3) and InsP(5) from Ins(1,3,4,5)P(4).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers
  • In Situ Hybridization
  • Inositol 1,4,5-Trisphosphate / biosynthesis*
  • Male
  • Molecular Sequence Data
  • Phosphotransferases (Alcohol Group Acceptor) / chemistry
  • Phosphotransferases (Alcohol Group Acceptor) / genetics
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Amino Acid

Substances

  • DNA Primers
  • Inositol 1,4,5-Trisphosphate
  • Phosphotransferases (Alcohol Group Acceptor)
  • inositol polyphosphate multikinase

Associated data

  • GENBANK/AY014898