A solid sample preparation method that reduces signal suppression effects in the MALDI analysis of peptides

M Z Wang; M C Fitzgerald

doi:10.1021/ac0009090

A solid sample preparation method that reduces signal suppression effects in the MALDI analysis of peptides

Anal Chem. 2001 Feb 1;73(3):625-31. doi: 10.1021/ac0009090.

Authors

M Z Wang¹, M C Fitzgerald

Affiliation

¹ Department of Chemistry, Duke University, Durham, North Carolina 27708, USA.

PMID: 11217772
DOI: 10.1021/ac0009090

Abstract

Here we report on the application of a solid-solid (SS) sample preparation protocol for the MALDI analysis of peptides and multicomponent peptide mixtures. Our results with a series of model peptides indicate that a SS MALDI sample preparation protocol is useful for the analysis of peptides in the 1-3 kDa mass range. MALDI mass spectra recorded for peptides in this size range using a SS sample preparation were of a quality comparable to spectra recorded using a conventional dried-droplet (DD) sample preparation. Our results with several model peptide mixtures indicate that one advantage of a SS sample preparation protocol for the MALDI analysis of peptides is that it can significantly reduce signal suppression effects in multicomponent mixtures. MALDI results obtained using a SS sample preparation protocol are also more reproducible than results obtained using a conventional DD sample preparation protocol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Molecular Sequence Data
Peptides / analysis*
Peptides / chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Peptides