A comprehensive view on the possibilities of the most recently developed chromatographic methods and emerging techniques in the analysis of pesticides glyphosate, glufosinate, bialaphos and their metabolites is presented. The state-of-the-art of the individual pre-treatment steps (extraction, pre-concentration, clean-up, separation, quantification) of the employed analytical methods for this group of chemicals is reviewed. The advantages and drawbacks of the described analytical methods are discussed and the present status and future trends are outlined.