Fine-mapping and construction of a bovine contig spanning the ovine callipyge locus

Mamm Genome. 2001 Feb;12(2):141-9. doi: 10.1007/s003350010248.

Abstract

The callipyge (CLPG) gene was fine-mapped by linkage analysis to a 4.6-cM chromosome interval on distal ovine OAR18q, flanked by microsatellite markers IDVGA30 and OY3. The OAR18q linkage map and human HSA14q transcript map were aligned by genotyping two bovine-hamster whole-genome radiation hybrid panels with the microsatellite markers, as well as with sequences corresponding to HSA 14q genes. Using Type I loci mapping to the IDVGA30-OY3 interval as anchor points, we have constructed a 1.4-Mb bovine BAC contig containing the IDVGA30-OY3 interval. We demonstrate that the IDVGA30-OY3 interval spans approximately 770 kb and contains at least four genes: YY1, WARS, DLK1, and GTL2.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Cloning, Molecular
  • Contig Mapping*
  • Cricetinae
  • Crosses, Genetic
  • Genotype
  • Male
  • Microsatellite Repeats / genetics
  • Muscle Development
  • Muscle Fibers, Fast-Twitch / metabolism
  • Muscle, Skeletal / growth & development
  • Muscle, Skeletal / metabolism
  • Proteins / genetics*
  • Radiation Hybrid Mapping
  • Sheep

Substances

  • Proteins