Abstract
Single cell cDNA libraries were constructed from taste bud cells of rat circumvallate papillae. Using three steps of screening, including differential hybridization, sequence analyses and in situ hybridization, a clone encoding a rat homolog of yeast adenylyl cyclase-associated protein (CAP) was identified to be highly expressed in a subset of taste bud cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antisense Elements (Genetics)
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Cell Cycle Proteins / genetics*
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Cytoskeletal Proteins*
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Drosophila Proteins*
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Gene Expression / physiology
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Gene Library
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In Situ Hybridization
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Microfilament Proteins / genetics
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Proteins*
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RNA, Messenger / analysis
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Rats
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Rats, Wistar
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Taste Buds / chemistry
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Taste Buds / cytology
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Taste Buds / physiology*
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Yeasts
Substances
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Antisense Elements (Genetics)
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Cap1 protein, rat
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Capt protein, Drosophila
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Cell Cycle Proteins
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Cytoskeletal Proteins
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Drosophila Proteins
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Microfilament Proteins
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Proteins
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RNA, Messenger