Abstract
A lymphoid-committed progenitor population was isolated from mouse bone marrow based on the cell surface phenotype Thy-1.1(neg)Sca-1(pos)c-Kit(low)Lin(neg). These cells were CD43(pos)CD24(pos) on isolation and proliferated in response to the cytokine combination of steel factor, IL-7, and Flt3 ligand. Lymphoid-committed progenitors could be segregated into more primitive and more differentiated subsets based on expression of AA4.1. The more differentiated subset generated only B lymphoid cells in 92% of total colonies assayed, lacked T lineage potential, and expressed Pax5. These studies have therefore defined and isolated a B lymphoid-committed progenitor population at a developmental stage corresponding to the initial expression of CD45R.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Aging / genetics
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Aging / immunology*
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Animals
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Antigens, Differentiation, B-Lymphocyte / biosynthesis
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B-Lymphocyte Subsets / cytology
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B-Lymphocyte Subsets / immunology*
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B-Lymphocyte Subsets / metabolism
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Bone Marrow Cells / cytology
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Bone Marrow Cells / immunology*
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Bone Marrow Cells / metabolism
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Cell Lineage / genetics
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Cell Lineage / immunology
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Cell Separation
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Cells, Cultured
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Gene Expression Regulation, Developmental / immunology
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Hyaluronan Receptors*
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Immunophenotyping*
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Membrane Glycoproteins*
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Mice
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Mice, Inbred AKR
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Mice, Inbred C57BL
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Mitochondrial Proteins
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Myeloid Cells / cytology
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Myeloid Cells / immunology
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Myeloid Cells / metabolism
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Proto-Oncogene Proteins c-kit / biosynthesis
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Receptors, Complement / biosynthesis
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Stem Cells / cytology
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Stem Cells / immunology*
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Stem Cells / metabolism
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Thy-1 Antigens / biosynthesis
Substances
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Antigens, Differentiation, B-Lymphocyte
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C1qbp protein, mouse
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Hyaluronan Receptors
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Membrane Glycoproteins
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Mitochondrial Proteins
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Receptors, Complement
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Thy-1 Antigens
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complement 1q receptor
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Proto-Oncogene Proteins c-kit