Synthesis of N-labeled peptidyl AMP

H Narita; T Moriguchi; K Seio; M Sekine; H Miyaguchi; K Sakamoto; S Yokoyama

doi:10.1080/15257770008045473

Synthesis of N-labeled peptidyl AMP

Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1993-2003. doi: 10.1080/15257770008045473.

Authors

H Narita¹, T Moriguchi, K Seio, M Sekine, H Miyaguchi, K Sakamoto, S Yokoyama

Affiliation

¹ Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.

PMID: 11200286
DOI: 10.1080/15257770008045473

Abstract

This paper deals with the synthesis of a new type of N-labeled peptidyl AMP, which would be used as a good substrate for analysis of the peptidyl transfer reaction on ribosome and for co-crystallization with ribosome. 4-(Dimethylamino)azobenzene-4'-sulfonyl (Dabsyl) was selected as the labeling group. (N-Dabsylglycyl)-L-leucyl AMP was synthesized from glycyl-L-leucine via a three-step procedure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Monophosphate / chemical synthesis*
Adenosine Monophosphate / chemistry
Peptides / chemistry*
Spectrum Analysis

Substances

Peptides
Adenosine Monophosphate