Predicting electrospray response from chromatographic retention time

N B Cech; J R Krone; C G Enke

doi:10.1021/ac0006019

Predicting electrospray response from chromatographic retention time

Anal Chem. 2001 Jan 15;73(2):208-13. doi: 10.1021/ac0006019.

Authors

N B Cech¹, J R Krone, C G Enke

Affiliation

¹ Department of Chemistry, The University of New Mexico, Albuquerque 87131-1096, USA.

PMID: 11199967
DOI: 10.1021/ac0006019

Abstract

The relationship between electrospray ionization response and HPLC retention time was explored. For the series of small peptides studied, higher ESI response was observed for analytes with longer reversed-phase HPLC retention times. This correlation existed for both experimentally measured retention times and those calculated from amino acid retention coefficients. This study is useful t

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Algorithms
Oligopeptides / chemistry*
Spectrometry, Mass, Electrospray Ionization / statistics & numerical data*

Substances

Oligopeptides

Grants and funding

GM 44077/GM/NIGMS NIH HHS/United States