Abstract
We investigated the suppressive effects of an avocado constituent, persenone A, on lipopolysaccharide- and interferon-y-induced inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in a mouse macrophage cell line RAW 264.7. Persenone A at concentration of 20 microM almost completely suppressed both iNOS and COX-2 protein expression. In mouse skin, double treatments with persenone A (810 nmol) significantly suppressed double 12-O-tetradecanoylphorbol-13-acetate (TPA, 8.1 nmol) application-induced hydrogen peroxide (H2O2) generation. Treatment with persenone A before the second TPA treatment was sufficient to inhibit H2O2 generation, while the first treatment was not. This study thus suggests that persenone A is a possible agent to prevent inflammation-associated diseases including cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Western
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Carcinogens / toxicity
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Enzyme Induction
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Esters / chemical synthesis
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Esters / chemistry
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Esters / pharmacology*
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Fatty Alcohols / chemical synthesis
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Fatty Alcohols / chemistry
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Fatty Alcohols / pharmacology*
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Female
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Gene Silencing / drug effects
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Hydrogen Peroxide / metabolism*
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Lauraceae / chemistry*
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Macrophages / drug effects*
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Macrophages / enzymology
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Mice
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Mice, Inbred ICR
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Nitric Oxide Synthase / genetics
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Nitric Oxide Synthase / metabolism*
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Nitric Oxide Synthase Type II
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Prostaglandin-Endoperoxide Synthases / genetics
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Prostaglandin-Endoperoxide Synthases / metabolism*
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Skin / drug effects*
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Skin / metabolism
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Tetradecanoylphorbol Acetate / toxicity
Substances
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Carcinogens
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Esters
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Fatty Alcohols
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persenone A
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Hydrogen Peroxide
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nos2 protein, mouse
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Prostaglandin-Endoperoxide Synthases
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Tetradecanoylphorbol Acetate