Abstract
The trehalose operon of Pseudomonas fluorescens ATCC 17400 consists of treP, treA and treR. The gene treP codes for a putative enzyme II subunit of the phosphotransferase system that catalyzes the phosphorylation of trehalose together with its translocation across the cell membrane and treA encodes a putative phosphotrehalase, which hydrolyzes the incoming trehalose-6-phosphate into glucose and glucose-6-phosphate. Both genes are negatively regulated by TreR, a repressor of the FadR-GntR family of transcription regulators. The operon that is induced by trehalose present in the medium shows a high similarity both in the function of genes and in the regulation with the trehalose operon of Bacillus subtilis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / genetics
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Base Sequence
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Cloning, Molecular
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Disaccharidases / genetics
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Disaccharidases / metabolism
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Genes, Bacterial*
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Genes, Regulator
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Molecular Sequence Data
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Mutation
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Operon
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Phosphoenolpyruvate Sugar Phosphotransferase System / genetics
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Pseudomonas fluorescens / enzymology
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Pseudomonas fluorescens / genetics*
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Sequence Alignment
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Transcription Factors / genetics
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Trehalose / genetics*
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beta-Galactosidase / analysis
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beta-Galactosidase / metabolism
Substances
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Bacterial Proteins
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TraR protein, Agrobacterium tumefaciens
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Transcription Factors
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Trehalose
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Phosphoenolpyruvate Sugar Phosphotransferase System
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phosphoenolpyruvate-trehalose phosphotransferase, bacteria
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Disaccharidases
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beta-Galactosidase
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alpha, alpha-phosphotrehalase