Crystallization and preliminary crystallographic studies of an antimicrobial protein from Pharbitis nil

S C Ha; K Min; J C Koo; Y Kim; D J Yun; M J Cho; K K Kim

doi:10.1107/s090744490001516x

Crystallization and preliminary crystallographic studies of an antimicrobial protein from Pharbitis nil

Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):263-5. doi: 10.1107/s090744490001516x.

Authors

S C Ha¹, K Min, J C Koo, Y Kim, D J Yun, M J Cho, K K Kim

Affiliation

¹ Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Chinju 660-701, South Korea.

PMID: 11173474
DOI: 10.1107/s090744490001516x

Abstract

An antimicrobial protein from seeds of Pharbitis nil (Pn-AMP) which shows an antifungal activity towards several agriculturally important plant pathogens has been crystallized in the presence of equimolar N-acetylglucosamine with sodium citrate as precipitant. The crystal belongs to the hexagonal space group P6(1)22 (or P6(5)22), with unit-cell parameters a = b = 29.33 (5), c = 133.44 (12) A. Native data were collected using a crystal at 100 K to a resolution of 1.78 A.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Anti-Infective Agents / chemistry*
Anti-Infective Agents / isolation & purification
Antifungal Agents / chemistry
Crystallization
Crystallography, X-Ray
Magnoliopsida / chemistry*
Molecular Sequence Data
Plant Proteins / chemistry*
Plant Proteins / isolation & purification
Seeds / chemistry
Sequence Alignment
Sequence Homology, Amino Acid

Substances

Anti-Infective Agents
Antifungal Agents
Plant Proteins