Construction and characterization of an ovine BAC contig spanning the callipyge locus

K Segers; D Vaiman; S Berghmans; T Shay; S Meyers; J Beever; N Cockett; M Georges; C Charlier

doi:10.1046/j.1365-2052.2000.00676.x

Construction and characterization of an ovine BAC contig spanning the callipyge locus

Anim Genet. 2000 Dec;31(6):352-9. doi: 10.1046/j.1365-2052.2000.00676.x.

Authors

K Segers¹, D Vaiman, S Berghmans, T Shay, S Meyers, J Beever, N Cockett, M Georges, C Charlier

Affiliation

¹ Department of Genetics, Faculty of Veterinary Medicine, University of Liège, Belgium.

PMID: 11167521
DOI: 10.1046/j.1365-2052.2000.00676.x

Abstract

We describe the construction of an ovine BAC contig spanning a 4.6 centimorgan (cM) chromosome segment known to contain the callipyge (CLPG) locus. The contig comprises 21 ovine BAC clones jointly covering approximately 900 kilobases (Kb). Two gaps in the BAC contig, spanning 10 and 7.5 Kb, respectively, were bridged by long range PCR. The corresponding chromosome region was shown to be characterized by an unusually low Kb to cM ratio (164 Kb/cM) and a high density of Not1 sites (1:126 Kb) possibly reflecting a high gene density in the corresponding chromosome region. Equivalent amplification of 64 sequence tagged sites spanning the corresponding region from homozygous +/+ and CLPG/CLPG individuals disproves the hypothesis of a major deletion causing the CLPG mutation.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Base Sequence
Chromosomes, Artificial, Bacterial*
DNA Primers
Electrophoresis, Gel, Pulsed-Field
Polymerase Chain Reaction
Sequence Tagged Sites
Sheep / genetics*

Substances

DNA Primers